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Product Info Summary
| SKU: | A08587-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PTGR1 Antibody Picoband®
SKU/Catalog Number
A08587-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTGR1 Antibody Picoband® catalog # A08587-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PTGR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08587-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PTGR1 recombinant protein (Position: T8-A329).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A08587-1 is reactive to PTGR1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
45470 MW
Background of PGR1
This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08587-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human A549 whole cell, human RT4 whole cell, human SiHa whole cell, human Hacat whole cell, human Caco-2 whole cell, human PC-3 whole cell
FCM: RT4 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTGR1 using anti-PTGR1 antibody (A08587-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human SiHa whole cell lysates,
Lane 6: human Hacat whole cell lysates,
Lane 7: human Caco-2 whole cell lysates,
Lane 8: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR1 antigen affinity purified polyclonal antibody (Catalog # A08587-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGR1 at approximately 36 kDa. The expected band size for PTGR1 is at 36 kDa.
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Figure 2. Flow Cytometry analysis of RT4 cells using anti-PTGR1 antibody (A08587-1).
Overlay histogram showing RT4 cells stained with A08587-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGR1 Antibody (A08587-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PTGR1 (Source: Uniprot.org, NCBI)
Gene Name
PTGR1
Full Name
Prostaglandin reductase 1
Weight
45470 MW
Superfamily
NADP-dependent oxidoreductase L4BD family
Alternative Names
Gap junction gamma-1 protein;Connexin-45;Cx45;Gap junction alpha-7 protein;GJC1;GJA7; PTGR1 DIG-1, LTB4DH, PGR1, ZADH3 prostaglandin reductase 1 prostaglandin reductase 1|15-oxoprostaglandin 13-reductase|D3T-inducible gene 1 protein|NAD(P)H-dependent alkenal/one oxidoreductase|NADP-dependent leukotriene B4 12-hydroxydehydrogenase|PRG-1|dithiolethione-inducible gene 1 protein|leukotriene B4 12-hydroxydehydrogenase|zinc binding alcohol dehydrogenase domain containing 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGR1, check out the PTGR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTGR1 Antibody Picoband® (A08587-1)
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