Click image to see more details
Product Info Summary
| SKU: | A02548-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PTGER3 Antibody Picoband®
SKU/Catalog Number
A02548-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTGER3 Antibody Picoband® catalog # A02548-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PTGER3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02548-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PTGER3 recombinant protein (Position: K2-R390).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02548-1 is reactive to PTGER3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
43.31kDa
Background of PTGER3
The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02548-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human K562 whole cell, human SH-SY5Y whole cell, human THP-1 whole cell
FCM: THP-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PTGER3 using anti-PTGER3 antibody (A02548-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER3 antigen affinity purified polyclonal antibody (Catalog # A02548-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER3 at approximately 48 kDa. The expected band size for PTGER3 is at 43 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of THP-1 cells using anti-PTGER3 antibody (A02548-1).
Overlay histogram showing THP-1 cells stained with A02548-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGER3 Antibody (A02548-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PTGER3 (Source: Uniprot.org, NCBI)
Gene Name
PTGER3
Full Name
Prostaglandin E2 receptor EP3 subtype
Weight
43.31kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
EP3; EP3EP3e; EP3-I; EP3-II; EP3-III; EP3-IV; MGC141828; MGC141829; MGC27302; PGE receptor EP3 subtype; PGE receptor, EP3 subtype; PGE2 receptor EP3 subtype; PGE2-R; prostaglandin E receotor EP3 subtype 3 isoform; prostaglandin E receptor 3 (subtype EP3); prostaglandin E2 receptor EP3 subtype; prostaglandin receptor (PGE-2); Prostanoid EP3 receptor; PTGER3 PTGER3 EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3-VI, EP3e, PGE2-R, lnc003875 prostaglandin E receptor 3 prostaglandin E2 receptor EP3 subtype|PGE receptor, EP3 subtype|PGE2 receptor EP3 subtype|prostaglandin E receptor 3 (subtype EP3)|prostaglandin receptor (PGE-2)|prostanoid EP3 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGER3, check out the PTGER3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGER3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTGER3 Antibody Picoband® (A02548-1)
Hello CJ!
No publications found for A02548-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PTGER3 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PTGER3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
