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Product Info Summary
| SKU: | A04844-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PSTPIP1 Antibody Picoband®
SKU/Catalog Number
A04844-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSTPIP1 Antibody Picoband® catalog # A04844-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PSTPIP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04844-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PSTPIP1 recombinant protein (Position: M1-D394). Human PSTPIP1 shares 87.3% amino acid (aa) sequence identity with mouse PSTPIP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04844-1 is reactive to PSTPIP1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
47.591kDa
Background of PSTPIP1
Proline-serine-threonine phosphatase-interacting protein 1 is an enzyme that in humans is encoded by the PSTPIP1 gene. This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04844-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell
FCM: JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PSTPIP1 using anti-PSTPIP1 antibody (A04844-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSTPIP1 antigen affinity purified polyclonal antibody (Catalog # A04844-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSTPIP1 at approximately 45 kDa. The expected band size for PSTPIP1 is at 48 kDa.
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Figure 2. Flow Cytometry analysis of JK cells using anti-PSTPIP1 antibody (A04844-1).
Overlay histogram showing JK cells stained with A04844-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSTPIP1 Antibody (A04844-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PSTPIP1 (Source: Uniprot.org, NCBI)
Gene Name
PSTPIP1
Full Name
Proline-serine-threonine phosphatase-interacting protein 1
Weight
47.591kDa
Alternative Names
CD2BP1CD2-binding protein 1; CD2BP1L; CD2BP1S; H-PIPPEST phosphatase-interacting protein 1; PAPASCD2 antigen-binding protein 1; proline-serine-threonine phosphatase interacting protein 1; proline-serine-threonine phosphatase-interacting protein 1; PSTPIPCD2 cytoplasmic tail-binding protein PSTPIP1 CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPAS, PSTPIP proline-serine-threonine phosphatase interacting protein 1 proline-serine-threonine phosphatase-interacting protein 1|CD2 -binding protein 1|CD2 cytoplasmic tail-binding protein|CD2-binding protein 1|PEST phosphatase-interacting protein 1|truncated proline-serine-threonine phosphatase interacting protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSTPIP1, check out the PSTPIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSTPIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSTPIP1 Antibody Picoband® (A04844-1)
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