Anti-PSMC2 Antibody Picoband™

Product Name

Anti-PSMC2 Antibody Picoband™

View all Proteasome 19S S7 Antibodies

SKU/Catalog Number

A09306-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PSMC2 Antibody Picoband™ catalog # A09306-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PSMC2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09306-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PSMC2 recombinant protein (Position: K34-N433).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09306-1 is reactive to PSMC2 in Human, Monkey

Applications

A09306-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

49 kDa

Calculated molecular weight

Background of Proteasome 19S S7

26S protease regulatory subunit 7, also known as 26S proteasome AAA-ATPase subunit Rpt1, is an enzyme that in humans is encoded by the PSMC2 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PSMC2 using anti-PSMC2 antibody (A09306-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC2 antigen affinity purified polyclonal antibody (Catalog # A09306-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC2 at approximately 49 kDa. The expected band size for PSMC2 is at 49 kDa.

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Figure 2. IF analysis of PSMC2 using anti-PSMC2 antibody (A09306-1).
PSMC2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMC2 Antibody (A09306-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PSMC2 (Source: Uniprot.org, NCBI)

Alternative Names

26S protease regulatory subunit 7; 26S proteasome AAA-ATPase subunit RPT1; MGC3004; MSS1ATPase, 2; proteasome (prosome, macropain) 26S subunit, ATPase, 2; Protein MSS1; putative protein product of Nbla10058 PSMC2 MSS1, Nbla10058, RPT1, S7 proteasome 26S subunit, ATPase 2 26S proteasome regulatory subunit 7|26S protease regulatory subunit 7|26S proteasome AAA-ATPase subunit RPT1|mammalian suppressor of sgv-1 of yeast|protease 26S subunit 7|proteasome (prosome, macropain) 26S subunit, ATPase, 2|putative protein product of Nbla10058|testis secretory sperm-binding protein Li 197a

For more info on PSMC2, check out the PSMC2 Infographic

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In this infographic, you will see the following information for PSMC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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