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Product Info Summary
| SKU: | A09943-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PSF2/GINS2 Antibody Picoband®
SKU/Catalog Number
A09943-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSF2/GINS2 Antibody Picoband® catalog # A09943-3. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PSF2/GINS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09943-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PSF2/GINS2 recombinant protein (Position: M1-F185). Human GINS2 shares 92.4% amino acid (aa) sequence identity with mouse GINS2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09943-3 is reactive to GINS2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
21 kDa
Background of PSF2
DNA replication complex GINS protein PSF2 is a protein that in humans is encoded by the GINS2 gene. The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611), Psf1 (GINS1; MIM 610608), Psf2, and Psf3 (GINS3; MIM 610610). The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09943-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human HepG2 whole cell, human 293T whole cell
FCM: A549 cell, JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PSF2/GINS2 using anti-PSF2/GINS2 antibody (A09943-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSF2/GINS2 antigen affinity purified polyclonal antibody (Catalog # A09943-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSF2/GINS2 at approximately 21 kDa. The expected band size for PSF2/GINS2 is at 21 kDa.
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Figure 2. Flow Cytometry analysis of A549 cells using anti-PSF2/GINS2 antibody (A09943-3).
Overlay histogram showing A549 cells stained with A09943-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSF2/GINS2 Antibody (A09943-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of JK cells using anti-PSF2/GINS2 antibody (A09943-3).
Overlay histogram showing JK cells stained with A09943-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSF2/GINS2 Antibody (A09943-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GINS2 (Source: Uniprot.org, NCBI)
Gene Name
GINS2
Full Name
DNA replication complex GINS protein PSF2
Weight
21 kDa
Superfamily
GINS2/PSF2 family
Alternative Names
DNA replication complex GINS protein PSF2; GINS complex subunit 2 (Psf2 homolog); GINS complex subunit 2; HSPC037; PSF2Pfs2 GINS2 HSPC037, PSF2, Pfs2 GINS complex subunit 2 DNA replication complex GINS protein PSF2|GINS complex subunit 2 (Psf2 homolog)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GINS2, check out the GINS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GINS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSF2/GINS2 Antibody Picoband® (A09943-3)
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