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Product Info Summary
| SKU: | A13674 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PRPF18/Prp18 Antibody
SKU/Catalog Number
A13674
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PRPF18/Prp18 Antibody catalog # A13674. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRPF18/Prp18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13674)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PRPF18. AA range:182-231
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13674 is reactive to PRPF18 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
39860 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13674 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293 cells using PRPF18 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using PRPF18 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PRPF18 (Source: Uniprot.org, NCBI)
Gene Name
PRPF18
Full Name
Pre-mRNA-splicing factor 18
Weight
39860 MW
Superfamily
PRP18 family
Alternative Names
Pre-mRNA-splicing factor 18;PRP18 homolog;hPRP18;PRPF18;HPRP18; PRPF18 PRP18, hPrp18 pre-mRNA processing factor 18 pre-mRNA-splicing factor 18|PRP18 homolog|PRP18 pre-mRNA processing factor 18 homolog|PRP18 pre-mRNA processing factor 18 homolog(PRPF18)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRPF18, check out the PRPF18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRPF18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRPF18/Prp18 Antibody (A13674)
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Customer Q&As
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6 Customer Q&As for Anti-PRPF18/Prp18 Antibody
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-PRPF18/Prp18 antibody A13674. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PRPF18/Prp18 antibody A13674. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-18
Question
Can you help my question with product A13674, anti-PRPF18/Prp18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13674 anti-PRPF18/Prp18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-01
Question
Does anti-PRPF18/Prp18 antibody A13674 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
According to the expression profile of cervix carcinoma, PRPF18 is highly expressed in cervix carcinoma. So, it is likely that anti-PRPF18/Prp18 antibody A13674 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-04-04
Question
We are currently using anti-PRPF18/Prp18 antibody A13674 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
B. Huang
Verified customer
Asked: 2014-03-24
Answer
The anti-PRPF18/Prp18 antibody (A13674) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-24
Question
Is this A13674 anti-PRPF18/Prp18 antibody reactive to the isotypes of PRPF18?
Z. Singh
Verified customer
Asked: 2013-09-26
Answer
The immunogen of A13674 anti-PRPF18/Prp18 antibody is Synthesized peptide derived from the Internal region of human PRPF18. at AA rangle: 160-240. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-09-26
