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Product Info Summary
| SKU: | A15188 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Protein yippee-like 5 YPEL5 Antibody
SKU/Catalog Number
A15188
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein yippee-like 5 YPEL5 Antibody (Catalog # A15188). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
YPEL5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein yippee-like 5 YPEL5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15188)
Host
Rabbit
Contents
YPEL5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
YPEL5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human YPEL5. The immunogen is located within the first 50 amino acids of YPEL5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
GLIPR1L1 antibody is human specific. At least two isoforms of GLIPR1L1 are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other GLIPR or GLIPR-like proteins.
Reactive Species
A15188 is reactive to YPEL5 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
13842 MW
Background of YPEL5
YPEL5 (yippee-like 5) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL5 is expressed at the nucleus and centrosome during interphase; during mitosis, it localizes to the spindle poles, mitotic spindle, and spindle midzone during mitosis. Finally, during cytokinesis, YPEL5 is localized to the midbody. It is associated with the Ran Binding Protein in the Microtubule organizing center (RanBPM) and a related protein RanBP10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15188 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
YPEL5 antibody can be used for detection of YPEL5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of YPEL5 in A-20 cell lysate with YPEL5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of YPEL5 in A20 cells with YPEL5 antibody at 2.5 μg/mL.
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Immunofluorescence of YPEL5 in A20 cells with YPEL5 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For YPEL5 (Source: Uniprot.org, NCBI)
Gene Name
YPEL5
Full Name
Protein yippee-like 5
Weight
13842 MW
Superfamily
yippee family
Alternative Names
CGI-127, Protein yippee-like 5 YPEL5 CGI-127 yippee like 5 protein yippee-like 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YPEL5, check out the YPEL5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YPEL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein yippee-like 5 YPEL5 Antibody (A15188)
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