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Product Info Summary
| SKU: | A01206 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein numb homolog NUMB Antibody
SKU/Catalog Number
A01206
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein numb homolog NUMB Antibody (Catalog # A01206). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NUMB antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein numb homolog NUMB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01206)
Host
Rabbit
Contents
NUMB Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NUMB antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human NUMB. The immunogen is located within the last 50 amino acids of NUMB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of NUMB are known to exist; this antibody will detect all of them.
Reactive Species
A01206 is reactive to NUMB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
70804 MW
Background of NUMB
NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01206 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NUMB antibody can be used for detection of NUMB by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 μg/mL.
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Immunohistochemistry of NUMB in human lung tissue with NUMB antibody at 5 μg/mL.
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Immunofluorescence of NUMB in human lung tissue with NUMB antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NUMB (Source: Uniprot.org, NCBI)
Gene Name
NUMB
Full Name
Protein numb homolog
Weight
70804 MW
Alternative Names
S171, C14orf41, c14_5527, Protein numb homolog, Protein S171, h-Numb NUMB C14orf41, S171, c14_5527 NUMB endocytic adaptor protein protein numb homolog|h-Numb|numb homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUMB, check out the NUMB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUMB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein numb homolog NUMB Antibody (A01206)
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