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Product Info Summary
| SKU: | A11542 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein dispatched homolog 1 DISP1 Antibody
SKU/Catalog Number
A11542
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein dispatched homolog 1 DISP1 Antibody (Catalog # A11542). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
DISP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein dispatched homolog 1 DISP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11542)
Host
Rabbit
Contents
DISP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DISP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human DISP1. The immunogen is located within amino acids 20 - 70 of DISP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of DISP1 are known to exist; this antibody will detect both isoforms. DISP1 antibody is predicted to not cross-react with EMX1.
Reactive Species
A11542 is reactive to DISP1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
170934 MW
Background of Disp1
DISP1 is the mammalian homolog of the Drosophila Dispatched segment-polarity gene and a key regulator of the Hedgehog (Hh) signaling pathway during embryonic development. DISP1 is required to move Shh from site of synthesis during embryogenesis; mutations in the gene result in lethality at mid-gestation and prevents specification of ventral cell types in the neural tube. Recent results have shown that DISP1 mediates the basolateral secretion of Shh and regulates growth of mammalian long bones through the control of Ihh.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11542 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DISP1 antibody can be used for detection of DISP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DISP1 in 3T3 cell lysate with DISP1 antibody at 1 μg/mL.
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Immunohistochemistry of DISP1 in human kidney tissue with DISP1 antibody at 2.5 μg/mL.
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Immunofluorescence of DISP1 in human kidney tissue with DISP1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DISP1 (Source: Uniprot.org, NCBI)
Gene Name
DISP1
Full Name
Protein dispatched homolog 1
Weight
170934 MW
Superfamily
dispatched family
Alternative Names
DISPA, DISPA, Protein dispatched homolog 1 Disp1|1190008H24Rik, D, DispA|dispatched RND transporter family member 1|protein dispatched homolog 1|dispatched A|dispatched homolog 1|mdispa|twelve-pass transmembrane protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DISP1, check out the DISP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DISP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein dispatched homolog 1 DISP1 Antibody (A11542)
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