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Product Info Summary
| SKU: | A05652 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Protein crumbs homolog 2 CRB2 Antibody
SKU/Catalog Number
A05652
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein crumbs homolog 2 CRB2 Antibody (Catalog # A05652). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CRB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Protein crumbs homolog 2 CRB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05652)
Host
Rabbit
Contents
CRB2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal CRB2 antibody was raised against a 17 amino acid peptide near the center of human CRB2. The immunogen is located within amino acids 460 - 510 of CRB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.
Reactive Species
A05652 is reactive to CRB2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
134265 MW
Background of Crb2
CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05652 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CRB2 antibody can be used for detection of CRB2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human,mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CRB2 in rat brain tissue lysate with CRB2 antibody at (A) 0.5 and (B) 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CRB2 (Source: Uniprot.org, NCBI)
Gene Name
CRB2
Full Name
Protein crumbs homolog 2
Weight
134265 MW
Superfamily
Crumbs protein family
Alternative Names
Protein crumbs homolog 2, Crumbs-like protein 2 Crb2|5930402A21, BC043114|crumbs family member 2|protein crumbs homolog 2|crumbs homolog 2|crumbs-like protein 2|crumbs2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRB2, check out the CRB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein crumbs homolog 2 CRB2 Antibody (A05652)
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