Anti-Protein crumbs homolog 2 CRB2 Antibody

Product Name

Anti-Protein crumbs homolog 2 CRB2 Antibody

View all Crb2 Antibodies

SKU/Catalog Number

A05652

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein crumbs homolog 2 CRB2 Antibody (Catalog # A05652). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CRB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Protein crumbs homolog 2 CRB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05652)

Host

Rabbit

Contents

CRB2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rabbit polyclonal CRB2 antibody was raised against a 17 amino acid peptide near the center of human CRB2. The immunogen is located within amino acids 460 - 510 of CRB2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.

Reactive Species

A05652 is reactive to CRB2 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

134265 MW

Background of Crb2

CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05652 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CRB2 antibody can be used for detection of CRB2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human,mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CRB2 (Source: Uniprot.org, NCBI)

Alternative Names

Protein crumbs homolog 2, Crumbs-like protein 2 Crb2|5930402A21, BC043114|crumbs family member 2|protein crumbs homolog 2|crumbs homolog 2|crumbs-like protein 2|crumbs2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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