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Product Info Summary
| SKU: | A02087 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein aurora borealis Bora Antibody
SKU/Catalog Number
A02087
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein aurora borealis Bora Antibody (Catalog # A02087). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Bora antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein aurora borealis Bora Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02087)
Host
Rabbit
Contents
Bora Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bora antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Bora. The immunogen is located within amino acids 410 - 460 of Bora.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.
Reactive Species
A02087 is reactive to BORA in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
61203 MW
Background of Bora
Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02087 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bora antibody can be used for detection of Bora by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bora in mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 μg/mL.
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Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BORA (Source: Uniprot.org, NCBI)
Gene Name
BORA
Full Name
Protein aurora borealis
Weight
61203 MW
Superfamily
BORA family
Alternative Names
C13orf34, C13orf34, Protein aurora borealis, HsBora
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BORA, check out the BORA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BORA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein aurora borealis Bora Antibody (A02087)
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