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Product Info Summary
| SKU: | A02001 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Prosurfactant Protein C/SFTPC Antibody Picoband®
View all Prosurfactant Protein C Antibodies
SKU/Catalog Number
A02001
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® catalog # A02001. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02001)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Prosurfactant Protein C/SFTPC recombinant protein (Position: S98-I193).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02001 is reactive to Sftpc in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
62616 MW
Background of Prosurfactant Protein C
SFTPC (Surfactant pulmonary -associated protein C), also known as SFTP2, SPC, PSP-C or PULMONARY SURFACTANT PROTEIN SP5, is a protein that in humans is encoded by the SFTPC gene. It is a membrane protein which manufactures surfactant. Glasser et al. (1988) assigned the SFTPC gene, symbolized SFTP2 by the Human Gene Mapping nomenclature committee, to chromosome 8 by hybridization to somatic cell hybrid DNA. Wood et al. (1994) identified 4 cosmids at the SFTP2 locus by cDNA hybridization. Using a polymorphic CA repeat in the SFTP2 gene and linkage analysis in 8 CEPH families, they located the gene close to D8S298; maximum lod = 19.3 at theta = 0.01. They regionalized the gene to 8p21 by fluorescence in situ hybridization. Moore et al. (1992) showed that the corresponding mouse gene, Sftp2, maps to chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02001 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Prosurfactant Protein C/SFTPC using anti-Prosurfactant Protein C/SFTPC antibody (A02001).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prosurfactant Protein C/SFTPC antigen affinity purified polyclonal antibody (Catalog # A02001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prosurfactant Protein C/SFTPC at approximately 21KD. The expected band size for Prosurfactant Protein C/SFTPC is at 21KD.
Protein Target Info & Infographic
Gene/Protein Information For Sftpc (Source: Uniprot.org, NCBI)
Gene Name
Sftpc
Full Name
Pulmonary surfactant-associated protein C
Weight
62616 MW
Alternative Names
Bifunctional epoxide hydrolase 2;Cytosolic epoxide hydrolase 2;CEH;3.3.2.10;Epoxide hydratase;Soluble epoxide hydrolase;SEH;Lipid-phosphate phosphatase;3.1.3.76;EPHX2; SFTPC BRICD6, PSP-C, SFTP2, SMDP2, SP-C, SP5 surfactant protein C pulmonary surfactant-associated protein C|BRICHOS domain containing 6|pulmonary surfactant apoprotein-2 SP-C|pulmonary surfactant-associated proteolipid SPL(Val)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sftpc, check out the Sftpc Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sftpc: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prosurfactant Protein C/SFTPC Antibody Picoband® (A02001)
Hello CJ!
A02001 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang L,Liu G,Fu L,Zhong W,Li X,Pan Q. DNA repair enzyme OGG1 promotes alveolar progenitor cell renewal and relieves PM2.5-induced lung injury and fibrosis. Ecotoxicol Environ Saf.2020 Dec 1;205:111283.doi: 10.1016/j.ecoenv.2020.111283. Epub 2020 Sep 22.PM
Species: Human,Mouse
A02001 usage in article: APP:IF, SAMPLE:LUNG TISSUE, DILUTION:1:50
Fan Y,Zhang G,Vong CT,Ye RD.Serum amyloid A3 confers protection against acute lung injury in Pseudomonas aeruginosa-infected mice. Am J Physiol Lung Cell Mol Physiol.2020 Feb 1;318(2):L314-L322.doi:10.1152/ ajplung.00309.2019.Epub 2019 Dec 18.PMID:3185153
Species: Mouse
A02001 usage in article: APP:IF, SAMPLE:LUNG TISSUE, DILUTION:1:500
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