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Product Info Summary
| SKU: | PA1583 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody Picoband®
SKU/Catalog Number
PA1583
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody catalog # PA1583. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1583)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Prostaglandin E Receptor EP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1583 is reactive to PTGER1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
41801 MW
Background of PTGER1
PTGER1, Prostaglandin E Receptor 1, comprises 402 amino acids with a predicted molecular mass of 41, 858 and has the 7 predicted transmembrane-spanning domains common to all G protein-coupled receptors. The PTGER1 gene is mapped to chromosome 19p13.1. The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). Through a phosphatidylinositol-calcium second messenger system, Gq proteins mediate this receptor's activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1583 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human 293T whole cell, human PC-3 whole cell, human HEL whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTGER1 using anti-PTGER1 antibody (PA1583).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER1 antigen affinity purified polyclonal antibody (Catalog # PA1583) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER1 at approximately 42 kDa. The expected band size for PTGER1 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PTGER1 (Source: Uniprot.org, NCBI)
Gene Name
PTGER1
Full Name
Prostaglandin E2 receptor EP1 subtype
Weight
41801 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Prostaglandin E2 receptor EP1 subtype;PGE receptor EP1 subtype;PGE2 receptor EP1 subtype;Prostanoid EP1 receptor;PTGER1; PTGER1 EP1 prostaglandin E receptor 1 prostaglandin E2 receptor EP1 subtype|PGE receptor, EP1 subtype|PGE2 receptor EP1 subtype|prostaglandin E receptor 1 (subtype EP1), 42kD|prostaglandin E receptor 1 (subtype EP1), 42kDa|prostaglandin E receptor 1, subtype EP1|prostanoid EP1 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGER1, check out the PTGER1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGER1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody Picoband® (PA1583)
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No publications found for PA1583
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Customer Q&As
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1 Customer Q&As for Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody Picoband®
Question
We are currently using anti-Prostaglandin E Receptor EP1/PTGER1 antibody PA1583 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
S. Lewis
Verified customer
Asked: 2013-04-23
Answer
The anti-Prostaglandin E Receptor EP1/PTGER1 antibody (PA1583) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-04-23
