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Product Info Summary
| SKU: | A00298-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Prolyl Endopeptidase/PREP Antibody Picoband®
View all Prolyl Oligopeptidase/PREP Antibodies
SKU/Catalog Number
A00298-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prolyl Endopeptidase/PREP Antibody Picoband® catalog # A00298-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prolyl Endopeptidase/PREP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00298-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PREP recombinant protein (Position: M1-E227).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00298-2 is reactive to PREP in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
81 kDa
Calculated molecular weight
50495 MW
Background of Prolyl Oligopeptidase/PREP
Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene. The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00298-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U937 whole cell, human HL-60 whole cell
IHC: human breast cancer tissue, human liver cancer tissue, human pancreatic cancer tissue, human prostate cancer tissue, human skin cancer tissue, human tonsil tissue, rat intestine tissue
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U937 whole cell lysates,
Lane 2: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolyl Endopeptidase/PREP antigen affinity purified polyclonal antibody (Catalog # A00298-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolyl Endopeptidase/PREP at approximately 81 kDa. The expected band size for Prolyl Endopeptidase/PREP is at 81 kDa.
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Figure 2. IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Prolyl Endopeptidase/PREP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. Flow Cytometry analysis of HL-60 cells using anti-Prolyl Endopeptidase/PREP antibody (A00298-2).
Overlay histogram showing HL-60 cells stained with A00298-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Prolyl Endopeptidase/PREP Antibody (A00298-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PREP (Source: Uniprot.org, NCBI)
Gene Name
PREP
Full Name
Prolyl endopeptidase
Weight
50495 MW
Superfamily
peptidase S9A family
Alternative Names
Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4; ERBB4 intracellular domain; 4ICD; E4ICD; s80HER4; ERBB4; HER4 PREP PE, PEP prolyl endopeptidase prolyl endopeptidase|dJ355L5.1 (prolyl endopeptidase)|post-proline cleaving enzyme|prolyl oligopeptidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PREP, check out the PREP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PREP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prolyl Endopeptidase/PREP Antibody Picoband® (A00298-2)
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