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Product Info Summary
| SKU: | PB9882 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Prokineticin 1/PROK1 Antibody Picoband®
View all EG-VEGF/PK1 Antibodies
SKU/Catalog Number
PB9882
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prokineticin 1/PROK1 Antibody Picoband® catalog # PB9882. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prokineticin 1/PROK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9882)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Prokineticin 1 recombinant protein (Position: A20-F105). Mouse Prokineticin 1 shares 88.4% and 94.2% amino acid (aa) sequence identity with human and rat Prokineticin 1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9882 is reactive to PROK1 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
11678 MW
Background of EG-VEGF/PK1
Prokineticin-1 is also known as EG-VEGF. The International Radiation Hybrid Mapping Consortium mapped the PROK1 gene to chromosome 1. The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9882 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse liver tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Prokineticin 1 using anti-Prokineticin 1 antibody (PB9882).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prokineticin 1 antigen affinity purified polyclonal antibody (Catalog # PB9882) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prokineticin 1 at approximately 12 kDa. The expected band size for Prokineticin 1 is at 12 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PROK1 (Source: Uniprot.org, NCBI)
Gene Name
PROK1
Full Name
Prokineticin-1
Weight
11678 MW
Superfamily
AVIT (prokineticin) family
Alternative Names
Prokineticin-1 ;Endocrine-gland-derived vascular endothelial growth factor ;EG-VEGF ;Prok1 ;Pk1 ; PROK1 EGVEGF, PK1, PRK1 prokineticin 1 prokineticin-1|EG-VEGF|black mamba toxin-related protein|endocrine-gland-derived vascular endothelial growth factor|mambakine
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROK1, check out the PROK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prokineticin 1/PROK1 Antibody Picoband® (PB9882)
Hello CJ!
PB9882 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Administration of pigment epithelium-derived factor delivered by adeno-associated virus inhibits blood?retinal barrier breakdown in diabetic rats
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Customer Q&As
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1 Customer Q&As for Anti-Prokineticin 1/PROK1 Antibody Picoband®
Question
We are currently using anti-Prokineticin 1/PROK1 antibody PB9882 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The anti-Prokineticin 1/PROK1 antibody (PB9882) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-22
