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Product Info Summary
| SKU: | PB9749 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Prealbumin/TTR Antibody Picoband®
View all Transthyretin/Prealbumin Antibodies
SKU/Catalog Number
PB9749
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prealbumin/TTR Antibody Picoband® catalog # PB9749. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prealbumin/TTR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9749)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Prealbumin recombinant protein (Position: G21-E147). Human Prealbumin shares 81% and 83.3% amino acid (aa) sequence identity with mouse and rat Prealbumin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9749 is reactive to TTR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated molecular weight
15887 MW
Background of Transthyretin/Prealbumin
Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9749 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA(Cap), 1-5 μg/ml, Human
Positive Control
WB: human placenta tissue, human HCCP tissue
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Prealbumin using anti-Prealbumin antibody (PB9749).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysate,
Lane 2: human HCCP tissue lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prealbumin antigen affinity purified polyclonal antibody (Catalog # PB9749) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prealbumin at approximately 14 kDa. The expected band size for Prealbumin is at 14 kDa.
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Figure 2. IHC analysis of Prealbumin using anti-Prealbumin antibody (PB9749).
Prealbumin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Prealbumin Antibody (PB9749) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Sandwich ELISA - Recombinant human IL15RA protein standard curve.
Use in combination with reagents from Human IL15RA ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1663).
Protein Target Info & Infographic
Gene/Protein Information For TTR (Source: Uniprot.org, NCBI)
Gene Name
TTR
Full Name
Transthyretin
Weight
15887 MW
Superfamily
transthyretin family
Alternative Names
Transthyretin;ATTR;Prealbumin;TBPA;TTR;PALB; TTR ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN transthyretin transthyretin|epididymis luminal protein 111|prealbumin, amyloidosis type I|thyroxine-binding prealbumin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTR, check out the TTR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prealbumin/TTR Antibody Picoband® (PB9749)
Hello CJ!
PB9749 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of renal cell markers and detection of 3p loss links endolymphatic sac tumor to renal cell carcinoma and warrants careful evaluation to avoid %u2026
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Prealbumin/TTR Antibody Picoband®
Question
My question regards using your anti-Prealbumin/TTR antibody for retinoid metabolic process studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We appreciate your inquiry. This PB9749 anti-Prealbumin/TTR antibody is validated on lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-10
Question
We ordered your anti-Prealbumin/TTR antibody for WB on liver in the past. I am using human, and We are going to use the antibody for IHC next. I am looking for examining liver as well as retina in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
G. Miller
Verified customer
Asked: 2019-10-04
Answer
I looked at the website and datasheets of our anti-Prealbumin/TTR antibody and it appears that PB9749 has been tested on human in both WB and IHC. Thus PB9749 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-04
Question
My team were well pleased with the WB result of your anti-Prealbumin/TTR antibody. However we have observed positive staining in brain secreted. cytoplasm. using this antibody. Is that expected? Could you tell me where is TTR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
From literature, brain does express TTR. Generally TTR expresses in secreted. cytoplasm. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2019-08-09
Question
We have observed staining in human cajal-retzius cell fetal brain cortex. What should we do? Is anti-Prealbumin/TTR antibody supposed to stain cajal-retzius cell fetal brain cortex positively?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
From what I have seen in literature cajal-retzius cell fetal brain cortex does express TTR. From what I have seen in Uniprot.org, TTR is expressed in pigmented layer of retina, liver, retina, corpus callosum, platelet, brain, cajal-retzius cell fetal brain cortex, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2019-07-04
Question
We are currently using anti-Prealbumin/TTR antibody PB9749 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
T. Evans
Verified customer
Asked: 2018-01-08
Answer
The anti-Prealbumin/TTR antibody (PB9749) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-08
