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Product Info Summary
| SKU: | A07113 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody
SKU/Catalog Number
A07113
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody (Catalog # A07113). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BCAS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07113)
Host
Rabbit
Contents
BCAS2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BCAS2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BCAS2. The immunogen is located within the last 50 amino acids of BCAS2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
GBP5 antibody is predicted to not cross-react with other GBP family members.
Reactive Species
A07113 is reactive to BCAS2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
26131 MW
Background of BCAS2
BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53-null or -mutant cells; this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07113 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BCAS2 antibody can be used for detection of BCAS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BCAS2 in MCF7 cell lysate with BCAS2 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of BCAS2 in human breast carcinoma with BCAS2 antibody at 5 μg/mL.
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Immunofluorescence of BCAS2 in human breast carcinoma tissue with BCAS2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BCAS2 (Source: Uniprot.org, NCBI)
Gene Name
BCAS2
Full Name
Pre-mRNA-splicing factor SPF27
Weight
26131 MW
Superfamily
SPF27 family
Alternative Names
DAM1, SPF27, Snt309, DAM1, Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2 BCAS2 DAM1, SPF27, Snt309 BCAS2 pre-mRNA processing factor pre-mRNA-splicing factor SPF27|DNA amplified in mammary carcinoma 1 protein|breast carcinoma amplified sequence 2|spliceosome associated protein, amplified in breast cancer|spliceosome-associated protein SPF 27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCAS2, check out the BCAS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCAS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody (A07113)
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