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Product Info Summary
| SKU: | A11092-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PRDM11 Antibody Picoband®
SKU/Catalog Number
A11092-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PRDM11 Antibody Picoband® catalog # A11092-2. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PRDM11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11092-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PRDM11 recombinant protein (Position: E37-L483). Human PRDM11 shares 89.7% amino acid (aa) sequence identity with mouse PRDM11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11092-2 is reactive to PRDM11 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
58 kDa
Background of PRDM11
PRDM11 (PR/SET Domain 11) is a Protein Coding gene. Diseases associated with PRDM11 include Hermansky-Pudlak Syndrome 1. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and methyltransferase activity. An important paralog of this gene is PRDM7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11092-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human A431 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue
ICC/IF: A549 cell
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRDM11 using anti-PRDM11 antibody (A11092-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM11 antigen affinity purified polyclonal antibody (Catalog # A11092-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM11 at approximately 58 kDa. The expected band size for PRDM11 is at 58 kDa.
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Figure 2. IF analysis of PRDM11 using anti-PRDM11 antibody (A11092-2).
PRDM11 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRDM11 Antibody (A11092-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A549 cells using anti-PRDM11 antibody (A11092-2).
Overlay histogram showing A549 cells stained with A11092-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM11 Antibody (A11092-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PRDM11 (Source: Uniprot.org, NCBI)
Gene Name
PRDM11
Full Name
PR domain-containing protein 11
Weight
58 kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
PFM8PR domain-containing protein 11; PR domain containing 11; PR-domain containing protein 11 PRDM11 PFM8 PR/SET domain 11 PR domain-containing protein 11|PR-domain containing protein 11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRDM11, check out the PRDM11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRDM11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRDM11 Antibody Picoband® (A11092-2)
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