Product Info Summary
SKU: | A06390-1 |
---|---|
Size: | 100μl |
Reactive Species: | Bovine, Human, Mouse, Rat, Zebrafish |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PPX PPP4C Monoclonal Antibody
SKU/Catalog Number
A06390-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PPX PPP4C Monoclonal Antibody catalog # A06390-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat, Zebrafish.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPX PPP4C Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06390-1)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
6C12
Isotype
IgG
Immunogen
Purified recombinant human PPX protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06390-1 is reactive to PPP4C in Bovine, Human, Mouse, Rat, Zebrafish
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
35080 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06390-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
IHC, 1:500-1:1000
IF, 1:100-1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Breast cancer using PPX Monoclonal antibody.
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Western Blot (WB) analysis using PPX Monoclonal antibody against HeLa, 293, Jurkat cell lysate.
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Immunofluorescence (IF) analysis of HeLa cells using PPX Monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PPP4C (Source: Uniprot.org, NCBI)
Gene Name
PPP4C
Full Name
Serine/threonine-protein phosphatase 4 catalytic subunit
Weight
35080 MW
Superfamily
PPP phosphatase family
Alternative Names
Serine/threonine-protein phosphatase 4 catalytic subunit;PP4C;Pp4;3.1.3.16;Protein phosphatase X;PP-X;PPP4C;PPP4, PPX; PPP4C PP-X, PP4, PP4C, PPH3, PPP4, PPX protein phosphatase 4 catalytic subunit serine/threonine-protein phosphatase 4 catalytic subunit|epididymis secretory sperm binding protein|protein phosphatase 4 (formerly X), catalytic subunit|protein phosphatase X, catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP4C, check out the PPP4C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP4C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPX PPP4C Monoclonal Antibody (A06390-1)
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No publications found for A06390-1
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PPX PPP4C Monoclonal Antibody
Question
We are currently using anti-PPX Monoclonal antibody A06390-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, human, mouse, rat, zebrafish. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-PPX Monoclonal antibody (A06390-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
Would anti-PPX Monoclonal antibody A06390-1 work on zebrafish IF with placenta?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
Our lab technicians have not validated anti-PPX Monoclonal antibody A06390-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PPX Monoclonal antibody A06390-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish placenta in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-PPX Monoclonal antibody A06390-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
you antibody to test anti-PPX Monoclonal antibody A06390-1 on rat blood for research purposes, then I may be interested in using anti-PPX Monoclonal antibody A06390-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
The products we sell, including anti-PPX Monoclonal antibody A06390-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-17
Question
Is this A06390-1 anti-PPX Monoclonal antibody reactive to the isotypes of PPP4C?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
The immunogen of A06390-1 anti-PPX Monoclonal antibody is Purified recombinant human PPX protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-22
Question
Please see the WB image, lot number and protocol we used for blood using anti-PPX Monoclonal antibody A06390-1. Please let me know if you require anything else.
R. Walker
Verified customer
Asked: 2013-02-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-14