Anti-PPP1R11 Antibody Picoband™

Product Name

Anti-PPP1R11 Antibody Picoband™

View all PPP1R11 Antibodies

SKU/Catalog Number

A10474-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPP1R11 Antibody Picoband™ catalog # A10474-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PPP1R11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10474-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PPP1R11 recombinant protein (Position: M1-H126).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10474-2 is reactive to PPP1R11 in Human, Mouse, Rat

Applications

A10474-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

41409 MW

Background of PPP1R11

Protein phosphatase 1 regulatory subunit 11 is an enzyme that in humans is encoded by the PPP1R11 gene. This gene encodes a specific inhibitor of protein phosphatase-1 (PP1) with a differential sensitivity toward the metal-independent and metal-dependent forms of PP1. The gene is located within the major histocompatibility complex class I region on chromosome 6.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PPP1R11 using anti-PPP1R11 antibody (A10474-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R11 antigen affinity purified polyclonal antibody (Catalog # A10474-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R11 at approximately 25 kDa. The expected band size for PPP1R11 is at 14,18,25-30 kDa.

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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PPP1R11 antibody (A10474-2).
Overlay histogram showing MCF-7 cells stained with A10474-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R11 Antibody (A10474-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PPP1R11 (Source: Uniprot.org, NCBI)

Alternative Names

HCG V; HCG-V; inhibitor-3; IPP3; protein phosphatase 1 regulatory subunit 11; protein phosphatase 1, regulatory (inhibitor) subunit 11; TCTE5; TCTEX5 PPP1R11 CFAP255, HCG-V, HCGV, IPP3, TCTE5, TCTEX5 protein phosphatase 1 regulatory inhibitor subunit 11 E3 ubiquitin-protein ligase PPP1R11|HCG V|hemochromatosis candidate gene V protein|inhibitor-3|protein phosphatase 1 regulatory subunit 11|protein phosphatase inhibitor 3|t-complex-associated-testis-expressed 5

For more info on PPP1R11, check out the PPP1R11 Infographic

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In this infographic, you will see the following information for PPP1R11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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