Anti-PPM1K/Pp2C Kappa Antibody

Product Name

Anti-PPM1K/Pp2C Kappa Antibody

View all PPM1K Antibodies

SKU/Catalog Number

A04319-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-PPM1K/Pp2C Kappa Antibody (Catalog# A04319-1). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PPM1K/Pp2C Kappa Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04319-1)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human PPM1K. Immunogen sequence location: 205 - 254

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04319-1 is reactive to PPM1K in Human, Mouse, Rat

Applications

A04319-1 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

40997 MW

Background of PPM1K

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Western blot analysis of lysates from 293 cells, using PPM1K Antibody. The lane on the right is blocked with the synthesized peptide.

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Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using PPM1K Antibody. The picture on the right is blocked with the synthesized peptide.

Gene/Protein Information For PPM1K (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp667B084; DKFZp761G058; mitochondrial; PP2C domain-containing protein phosphatase 1K; PP2Ckappa; PP2C-like mitochondrial protein; PP2CM; PP2C-type mitochondrial phosphoprotein phosphatase; protein phosphatase 2C kappa; protein phosphatase, Mg2+/Mn2+ dependent, 1K; PTMPEC 3.1.3.16 PPM1K BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07 protein phosphatase, Mg2+/Mn2+ dependent 1K protein phosphatase 1K, mitochondrial|PP2C domain-containing protein phosphatase 1K|PP2C-kappa|PP2C-type mitochondrial phosphoprotein phosphatase|branched-chain I+/--ketoacid dehydrogenase phosphatase|branched-chain alpha-ketoacid dehydrogenase phosphatase|protein phosphatase 2C kappa

For more info on PPM1K, check out the PPM1K Infographic

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In this infographic, you will see the following information for PPM1K: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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