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Product Info Summary
| SKU: | A04319-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-PPM1K/Pp2C Kappa Antibody
SKU/Catalog Number
A04319-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PPM1K/Pp2C Kappa Antibody (Catalog# A04319-1). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPM1K/Pp2C Kappa Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04319-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PPM1K. Immunogen sequence location: 205 - 254
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04319-1 is reactive to PPM1K in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
40997 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04319-1 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from 293 cells, using PPM1K Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using PPM1K Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PPM1K (Source: Uniprot.org, NCBI)
Gene Name
PPM1K
Full Name
Protein phosphatase 1K, mitochondrial
Weight
40997 MW
Superfamily
PP2C family
Alternative Names
PPM1K;PP2CM;Protein phosphatase 1K; mitochondrial;PP2C domain-containing protein phosphatase 1K;PP2C-like mitochondrial protein;PP2C-type mitochondrial phosphoprotein phosphatase;PTMP;Protein phosphatase 2C isoform kappa;PP2C-kappa PPM1K BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07 protein phosphatase, Mg2+/Mn2+ dependent 1K protein phosphatase 1K, mitochondrial|PP2C domain-containing protein phosphatase 1K|PP2C-kappa|PP2C-type mitochondrial phosphoprotein phosphatase|branched-chain I+/--ketoacid dehydrogenase phosphatase|branched-chain alpha-ketoacid dehydrogenase phosphatase|protein phosphatase 2C kappa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPM1K, check out the PPM1K Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPM1K: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPM1K/Pp2C Kappa Antibody (A04319-1)
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No publications found for A04319-1
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Customer Q&As
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2 Customer Q&As for Anti-PPM1K/Pp2C Kappa Antibody
Question
Is this A04319-1 anti-PPM1K/Pp2C Kappa antibody reactive to the isotypes of PPM1K?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
The immunogen of A04319-1 anti-PPM1K/Pp2C Kappa antibody is A peptide derived from human PPM1K. Immunogen sequence location: 205 - 254. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-24
Question
We are currently using anti-PPM1K/Pp2C Kappa antibody A04319-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
B. Moore
Verified customer
Asked: 2017-08-22
Answer
The anti-PPM1K/Pp2C Kappa antibody (A04319-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-22
