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Product Info Summary
| SKU: | A14264 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-PPAPDC1A PLPP4 Antibody
SKU/Catalog Number
A14264
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PPAPDC1A PLPP4 Antibody (Catalog # A14264). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PPAPDC1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PPAPDC1A PLPP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14264)
Host
Rabbit
Contents
PPAPDC1A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PPAPDC1A antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PPAPDC1A. The immunogen is located within the last 50 amino acids of PPAPDC1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
CHADL antibody is human, mouse and rat reactive. CHADL antibody is predicted to not cross-react with Chondroadherin protein.
Reactive Species
A14264 is reactive to PPAPDC1A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
30395 MW
Background of PLPP4
Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide(NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. PPAPDC1A is preferentially expressed in endothelial cells. Studies of PPAPDC1A and PAP activity suggest that they may play a role in angiogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14264 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PPAPDC1A antibody can be used for detection of PPAPDC1A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PPAPDC1A (Source: Uniprot.org, NCBI)
Gene Name
PPAPDC1A
Full Name
Phospholipid phosphatase 4
Weight
30395 MW
Superfamily
PA-phosphatase related phosphoesterase family
Alternative Names
DPPL2, PPAPDC1, DPPL2, Phosphatidate phosphatase PPAPDC1A, Phosphatidic acid phosphatase type 2 domain-containing protein 1A PLPP4 DPPL2, PPAPDC1, PPAPDC1A phospholipid phosphatase 4 phospholipid phosphatase 4|diacylglycerol pyrophosphate like 2|diacylglycerol pyrophosphate phosphatase-like 2|phosphatidate phosphatase PPAPDC1A|phosphatidic acid phosphatase type 2 domain containing 1A|phosphatidic acid phosphatase type 2 domain-containing protein 1A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPAPDC1A, check out the PPAPDC1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPAPDC1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPAPDC1A PLPP4 Antibody (A14264)
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