Click image to see more details
Product Info Summary
| SKU: | A10973 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PP14C PPP1R14C Antibody
SKU/Catalog Number
A10973
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PP14C PPP1R14C Antibody (Catalog # A10973). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP14C PPP1R14C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10973)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PP14C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10973 is reactive to PPP1R14C in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
17843 MW
Background of Ppp1r14c
Inhibitor of the PP1 regulatory subunit PPP1CA.
Scanlan M.J., Cancer Immun. 1:4-4(2001).
Liu Q.-R., J. Biol. Chem. 277:13312-13320(2002).
Mungall A.J., Nature 425:805-811(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10973 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells and COLO205 cells, using PP14C antibody A10973.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R14C (Source: Uniprot.org, NCBI)
Gene Name
PPP1R14C
Full Name
Protein phosphatase 1 regulatory subunit 14C
Weight
17843 MW
Superfamily
PP1 inhibitor family
Alternative Names
Protein phosphatase 1 regulatory subunit 14C;Kinase-enhanced PP1 inhibitor;PKC-potentiated PP1 inhibitory protein;Serologically defined breast cancer antigen NY-BR-81;PPP1R14C;KEPI; Ppp1r14c|6330514J04Rik, KE, KEPI|protein phosphatase 1, regulatory inhibitor subunit 14C|protein phosphatase 1 regulatory subunit 14C|PKC-potentiated PP1 inhibitory protein|kinase-enhanced PP1 inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R14C, check out the PPP1R14C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R14C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP14C PPP1R14C Antibody (A10973)
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No publications found for A10973
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PP14C PPP1R14C Antibody
Question
We are currently using anti-PP14C antibody A10973 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-PP14C antibody (A10973) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
I was wanting to use to test anti-PP14C antibody A10973 on mouse mammary gland for research purposes, then I may be interested in using anti-PP14C antibody A10973 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The products we sell, including anti-PP14C antibody A10973, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-04
Question
Can you help my question with product A10973, anti-PP14C antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10973 anti-PP14C antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-18
Question
Is a blocking peptide available for product anti-PP14C antibody (A10973)?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
We do provide the blocking peptide for product anti-PP14C antibody (A10973). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-27
Question
I see that the anti-PP14C antibody A10973 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-25
Question
Is there a BSA free version of anti-PP14C antibody A10973 available?
J. Evans
Verified customer
Asked: 2014-10-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PP14C antibody A10973 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-14
Question
Will anti-PP14C antibody A10973 work on feline WB with skin?
A. Jones
Verified customer
Asked: 2014-01-03
Answer
Our lab technicians have not validated anti-PP14C antibody A10973 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PP14C antibody A10973 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-03
