PPP1R14C Colorimetric Cell-Based ELISA

Ppp1r14c ELISA kit, cell-based

The PPP1R14C Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PPP1R14C protein expression profile in cells. The kit can be used for measuring the relative amounts of PPP1R14C in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PPP1R14C.

Product Info Summary

SKU: EKC1877
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

PPP1R14C Colorimetric Cell-Based ELISA

See all Ppp1r14c products

SKU/Catalog Number

EKC1877

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The PPP1R14C Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PPP1R14C protein expression profile in cells. The kit can be used for measuring the relative amounts of PPP1R14C in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PPP1R14C.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

PPP1R14C Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1877)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1877 is reactive to PPP1R14C in Human, Mouse, Rat samples

Application Guarantee

EKC1877 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-PPP1R14C Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For PPP1R14C (Source: Uniprot.Org, NCBI)

Gene Name

PPP1R14C

Full Name

Protein phosphatase 1 regulatory subunit 14C

Weight

17843 MW

Superfamily

PP1 inhibitor family

Alternative Names

Protein phosphatase 1 regulatory subunit 14C;Kinase-enhanced PP1 inhibitor;PKC-potentiated PP1 inhibitory protein;Serologically defined breast cancer antigen NY-BR-81;PPP1R14C;KEPI; Ppp1r14c|6330514J04Rik, KE, KEPI|protein phosphatase 1, regulatory inhibitor subunit 14C|protein phosphatase 1 regulatory subunit 14C|PKC-potentiated PP1 inhibitory protein|kinase-enhanced PP1 inhibitor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PPP1R14C, check out the PPP1R14C Infographic

PPP1R14C infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PPP1R14C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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