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Product Info Summary
| SKU: | A10549-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-POLR1A Antibody Picoband®
SKU/Catalog Number
A10549-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-POLR1A Antibody Picoband® catalog # A10549-1. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-POLR1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10549-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human POLR1A recombinant protein (Position: E607-R1720). Human POLR1A shares 86.3% and 87.3% amino acid (aa) sequence identity with mouse and rat POLR1A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10549-1 is reactive to POLR1A in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
194.11kDa
Background of Polr1a
DNA-directed RNA polymerase I subunit RPA1 is an enzyme that in humans is encoded by the POLR1A gene. The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10549-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POLR1A using anti-POLR1A antibody (A10549-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1A antigen affinity purified polyclonal antibody (A10549-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1A at approximately 195 kDa. The expected band size for POLR1A is at 195 kDa.
Protein Target Info & Infographic
Gene/Protein Information For POLR1A (Source: Uniprot.org, NCBI)
Gene Name
POLR1A
Full Name
DNA-directed RNA polymerase I subunit RPA1
Weight
194.11kDa
Superfamily
RNA polymerase beta' chain family
Alternative Names
DNA-directed RNA polymerase I subunit RPA1; A190; polymerase (RNA) I polypeptide A, 194kDa; RPA1; RPA194; RPO14; RPO1-4 Polr1a|194kD, 194kDa, 2900087K15Rik, 3010014K16Rik, RPA, RPA194, Rpo, Rpo1-4, mR, mRPA1|polymerase (RNA) I polypeptide A|DNA-directed RNA polymerase I subunit RPA1|DNA-directed RNA polymerase I largest subunit|DNA-directed RNA polymerase I subunit A|RNA polymerase 1-4 (194 kDa subunit)|RNA polymerase I 194 kDa subunit|RNA polymerase I subunit A1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR1A, check out the POLR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR1A Antibody Picoband® (A10549-1)
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