Product Info Summary
SKU: | A30700 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-POFUT1 Antibody
View all Protein O-Fucosyltransferase 1/POFUT1 Antibodies
SKU/Catalog Number
A30700
Size
100ul
Form
Liquid
Description
Boster Bio Anti-POFUT1 Antibody (Catalog# A30700). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POFUT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30700)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human POFUT1. Immunogen sequence location: 331-380
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30700 is reactive to POFUT1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
43956 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30700 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from Jurkat, HeLa, and HepG2 cells, using POFUT1 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using POFUT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For POFUT1 (Source: Uniprot.org, NCBI)
Gene Name
POFUT1
Full Name
GDP-fucose protein O-fucosyltransferase 1
Weight
43956 MW
Superfamily
glycosyltransferase 65 family
Alternative Names
POFUT1;FUT12;KIAA0180;GDP-fucose protein O-fucosyltransferase 1;Peptide-O-fucosyltransferase 1;O-FucT-1 POFUT1 DDD2, FUT12, O-FUT, O-Fuc-T, O-FucT-1, OFUCT1 protein O-fucosyltransferase 1 GDP-fucose protein O-fucosyltransferase 1|o-fucosyltransferase protein|peptide-O-fucosyltransferase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POFUT1, check out the POFUT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POFUT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POFUT1 Antibody (A30700)
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No publications found for A30700
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Customer Q&As
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1 Customer Q&As for Anti-POFUT1 Antibody
Question
We are currently using anti-POFUT1 antibody A30700 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The anti-POFUT1 antibody (A30700) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-01