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Product Info Summary
| SKU: | A00988-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PNP Antibody Picoband®
View all Purine Nucleoside Phosphorylase/PNP Antibodies
SKU/Catalog Number
A00988-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PNP Antibody Picoband® catalog # A00988-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PNP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00988-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PNP recombinant protein (Position: Q44-Q273). Human PNP shares 86.5% and 87% amino acid (aa) sequence identity with mouse and rat PNP, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00988-2 is reactive to PNP in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
32 kDa
Calculated molecular weight
32.118kDa
Background of Purine Nucleoside Phosphorylase/PNP
The PNP gene encodes purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T?lymphocyte function and neurologic dysfunction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00988-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human U-937 whole cell, rat PC-12 whole cell, rat NRK whole cell, mouse RAW2647 whole cell, mouse ANA-1 whole cell
FCM: JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PNP using anti-PNP antibody (A00988-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human U-937 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNP antigen affinity purified polyclonal antibody (Catalog # A00988-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNP at approximately 32 kDa. The expected band size for PNP is at 32 kDa.
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Figure 2. Flow Cytometry analysis of JK cells using anti-PNP antibody (A00988-2).
Overlay histogram showing JK cells stained with A00988-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (A00988-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PNP (Source: Uniprot.org, NCBI)
Gene Name
PNP
Full Name
Purine nucleoside phosphorylase
Weight
32.118kDa
Superfamily
PNP/MTAP phosphorylase family
Alternative Names
EC 2.4.2.1; FLJ94043; FLJ97288; Inosine phosphorylase; MGC117396; MGC125915; MGC125916; NPFLJ97312; nucleoside phosphorylase; PNP; PRO1837; PUNP; purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase PNP NP, PRO1837, PUNP purine nucleoside phosphorylase purine nucleoside phosphorylase|HEL-S-156an|epididymis secretory sperm binding protein Li 156an|inosine phosphorylase|inosine-guanosine phosphorylase|purine-nucleoside:orthophosphate ribosyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PNP, check out the PNP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PNP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PNP Antibody Picoband® (A00988-2)
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