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Product Info Summary
| SKU: | M01028-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PMS2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01028-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PMS2 Rabbit Monoclonal Antibody catalog # M01028-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PMS2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01028-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PMS2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01028-1 is reactive to PMS2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
95797 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01028-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human U20S whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PMS2 using anti-PMS2 antibody (M01028-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PMS2 antigen affinity purified monoclonal antibody (Catalog # M01028-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMS2 at approximately 120 kDa. The expected band size for PMS2 is at 96 kDa.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using PMS2 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
Protein Target Info & Infographic
Gene/Protein Information For PMS2 (Source: Uniprot.org, NCBI)
Gene Name
PMS2
Full Name
Mismatch repair endonuclease PMS2
Weight
95797 MW
Superfamily
DNA mismatch repair MutL/HexB family
Alternative Names
Mismatch repair endonuclease PMS2 ;3.1.-.-;DNA mismatch repair protein PMS2;PMS1 protein homolog 2;PMS2 ;PMSL2 ; PMS2 HNPCC4, MLH4, MMRCS4CL, PMSL2, PMS2 PMS1 homolog 2, mismatch repair system component mismatch repair endonuclease PMS2|DNA mismatch repair protein PMS2|PMS1 homolog 2, mismatch repair protein|PMS1 protein homolog 2|PMS2 postmeiotic segregation increased 2|postmeiotic segregation increased 2 nirs variant 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PMS2, check out the PMS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PMS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PMS2 Rabbit Monoclonal Antibody (M01028-1)
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4 Customer Q&As for Anti-PMS2 Rabbit Monoclonal Antibody
Question
My lab would like to test anti-PMS2 Rabbit Monoclonal antibody M01028-1 on human kidney for research purposes, then I may be interested in using anti-PMS2 Rabbit Monoclonal antibody M01028-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The products we sell, including anti-PMS2 Rabbit Monoclonal antibody M01028-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-27
Question
Will M01028-1 anti-PMS2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
It shows on the product datasheet, M01028-1 anti-PMS2 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-18
Question
I was wanting to use your anti-PMS2 Rabbit Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
J. Miller
Verified customer
Asked: 2017-07-27
Answer
It shows on the product datasheet, M01028-1 anti-PMS2 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-27
Question
Does anti-PMS2 Rabbit Monoclonal antibody M01028-1 work on feline IHC with amygdala?
S. Jackson
Verified customer
Asked: 2015-01-01
Answer
Our lab technicians have not validated anti-PMS2 Rabbit Monoclonal antibody M01028-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PMS2 Rabbit Monoclonal antibody M01028-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline amygdala in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-01
