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Product Info Summary
| SKU: | M01028 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PMS2 Monoclonal Antibody
SKU/Catalog Number
M01028
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PMS2 Monoclonal Antibody (Catalog # M01028). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-PMS2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01028)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 1204
Isotype
IgG1 kappa
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human PMS2 corresponding to the first 133 amino acid residues of the protein. The clone was produced using conventional hybridoma technology.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01028 is reactive to PMS2 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
95797 MW
Background of PMS2
PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01028 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Western blot analysis of PMS2 expression in H157 cell lysates 5μg(lane 1), H157 cell lysates 10μg (lane 2) and H157 cell lysates 30μg (lane 3). PMS2 at 96KD was detected using mouse anti-PMS2 Protein A purified monoclonal antibody (Catalog # M01028) at 1:1000. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignalsubstrate. Personal communication Morphotek Inc.
Protein Target Info & Infographic
Gene/Protein Information For PMS2 (Source: Uniprot.org, NCBI)
Gene Name
PMS2
Full Name
Mismatch repair endonuclease PMS2
Weight
95797 MW
Superfamily
DNA mismatch repair MutL/HexB family
Alternative Names
PMS2, DNA mismatch repair gene homologue, H_DJ0042M02.9, HNPCC4, PMS 2, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2, PMS2CL, PMSL2 PMS2 HNPCC4, MLH4, MMRCS4CL, PMSL2, PMS2 PMS1 homolog 2, mismatch repair system component mismatch repair endonuclease PMS2|DNA mismatch repair protein PMS2|PMS1 homolog 2, mismatch repair protein|PMS1 protein homolog 2|PMS2 postmeiotic segregation increased 2|postmeiotic segregation increased 2 nirs variant 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PMS2, check out the PMS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PMS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PMS2 Monoclonal Antibody (M01028)
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