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Product Info Summary
| SKU: | A31792 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PMIS2 Antibody Picoband®
SKU/Catalog Number
A31792
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PMIS2 Antibody Picoband® catalog # A31792. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PMIS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31792)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PMIS2 recombinant protein (Position: A47-R128).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A31792 is reactive to PMIS2 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
14377 MW
Background of Pmis2
PMIS2 might function both as the ultimate factor regulating sperm transport into the oviducts and in modulating sperm–zona binding. Predicted to act upstream of or within binding activity of sperm to zona pellucida. Predicted to be integral component of membrane. Predicted to be active in intracellular membrane-bounded organelle and membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31792 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human U251 whole cell, human HepG2 whole cell, human Caco-2 whole cell, rat C6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PMIS2 using anti-PMIS2 antibody (A31792).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMIS2 antigen affinity purified polyclonal antibody (Catalog # A31792) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMIS2 at approximately 19 kDa. The expected band size for PMIS2 is at 16 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PMIS2 (Source: Uniprot.org, NCBI)
Gene Name
PMIS2
Full Name
Transmembrane protein PMIS2
Weight
14377 MW
Superfamily
CD225/Dispanin family
Alternative Names
Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PMIS2, check out the PMIS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PMIS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PMIS2 Antibody Picoband® (A31792)
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