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Product Info Summary
| SKU: | M06377 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Plexin A1 Monoclonal Antibody
SKU/Catalog Number
M06377
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Plexin A1 Monoclonal Antibody catalog # M06377. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plexin A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06377)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFOO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Plexin A1 Plays a role in axon guidance, invasive growth and cell migration.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06377 is reactive to PLXNA1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
72 kDa
Calculated molecular weight
104738 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06377 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HUVEC cell
Validation Images & Assay Conditions
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Western blot analysis of Plexin A1 expression in HUVEC cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PLXNA1 (Source: Uniprot.org, NCBI)
Gene Name
PLXNA1
Full Name
Plexin-A1
Weight
104738 MW
Superfamily
plexin family
Alternative Names
Type II inositol 3,4-bisphosphate 4-phosphatase;3.1.3.66;Inositol polyphosphate 4-phosphatase type II;INPP4B; PLXNA1 NOV, NOVP, PLEXIN-A1, PLXN1 plexin A1 plexin-A1|plexin 1|semaphorin receptor NOV
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLXNA1, check out the PLXNA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLXNA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Plexin A1 Monoclonal Antibody (M06377)
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No publications found for M06377
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Customer Q&As
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6 Customer Q&As for Anti-Plexin A1 Monoclonal Antibody
Question
I am looking for to test anti-Plexin A1 Monoclonal antibody M06377 on human middle temporal gyrus for research purposes, then I may be interested in using anti-Plexin A1 Monoclonal antibody M06377 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
The products we sell, including anti-Plexin A1 Monoclonal antibody M06377, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-08
Question
Is a blocking peptide available for product anti-Plexin A1 Monoclonal antibody (M06377)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We do provide the blocking peptide for product anti-Plexin A1 Monoclonal antibody (M06377). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-01
Question
I was wanting to use your anti-Plexin A1 Monoclonal antibody for IF for human middle temporal gyrus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human middle temporal gyrus identification?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
You can see on the product datasheet, M06377 anti-Plexin A1 Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human middle temporal gyrus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-28
Question
Will anti-Plexin A1 Monoclonal antibody M06377 work for IF with middle temporal gyrus?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
According to the expression profile of middle temporal gyrus, PLXNA1 is highly expressed in middle temporal gyrus. So, it is likely that anti-Plexin A1 Monoclonal antibody M06377 will work for IF with middle temporal gyrus.
Boster Scientific Support
Answered: 2018-06-06
Question
Will M06377 anti-Plexin A1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Walker
Verified customer
Asked: 2017-01-27
Answer
It shows on the product datasheet, M06377 anti-Plexin A1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-27
Question
We are currently using anti-Plexin A1 Monoclonal antibody M06377 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
K. Krishna
Verified customer
Asked: 2013-12-18
Answer
The anti-Plexin A1 Monoclonal antibody (M06377) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-18
