Anti-Phospholipase D1/PLD1 Antibody Picoband®

Product Name

Anti-Phospholipase D1/PLD1 Antibody Picoband®

View all PLD1 Antibodies

SKU/Catalog Number

PB9380

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Phospholipase D1/PLD1 Antibody Picoband® catalog # PB9380. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospholipase D1/PLD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9380)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PLD1 recombinant protein (Position: M1-H330). Human PLD1 shares 91.8% and 90.9% amino acid (aa) sequence identity with mouse and rat PLD1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9380 is reactive to PLD1 in Human, Mouse

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

124 kDa

Calculated molecular weight

124184 MW

Background of PLD1

By somatic cell hybrid analysis, PLD1 is mapped to 3q26.31. This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. And PLD1 disrupted association of gamma-secretase protein components, independent of PLD1catalytic activity. What's more, PLD1 regulates intracellular trafficking of beta-amyloid, distinct from its effect on gamma-secretase activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9380 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Positive Control

WB: Mouse Cardiac Muscle Tissue, 22RV1 Whole Cell, HELA Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For PLD1 (Source: Uniprot.org, NCBI)

Alternative Names

Phospholipase D1;PLD 1;hPLD1;3.1.4.4;Choline phosphatase 1;Phosphatidylcholine-hydrolyzing phospholipase D1;PLD1; PLD1 CVDD phospholipase D1 phospholipase D1|choline phosphatase 1|phosphatidylcholine-hydrolyzing phospholipase D1|phospholipase D1, phosphatidylcholine-specific

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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