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Product Info Summary
| SKU: | A09775-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PKN3 Antibody Picoband®
SKU/Catalog Number
A09775-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKN3 Antibody Picoband® catalog # A09775-1. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PKN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09775-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PKN3 recombinant protein (Position: E2-P889). Human PKN3 shares 83.7% amino acid (aa) sequence identity with mouse PKN3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09775-1 is reactive to PKN3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
99 kDa
Calculated molecular weight
99 kDa
Background of PKN3
Protein kinase N3 is a serine/threonine protein kinase and one of three PKN isoforms (PKN1, PKN2, PKN3) encoded by independent genes. PKN3 has been identified as an effector that mediates malignant prostate cell growth and is activated by PI3-kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09775-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human K562 whole cell, human 293T whole cell, human HepG2 whole cell
ICC/IF: HELA cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PKN3 using anti-PKN3 antibody (A09775-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKN3 antigen affinity purified polyclonal antibody (Catalog # A09775-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKN3 at approximately 99 kDa. The expected band size for PKN3 is at 99 kDa.
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Figure 2. IF analysis of PKN3 using anti-PKN3 antibody (A09775-1).
PKN3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PKN3 Antibody (A09775-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-PKN3 antibody (A09775-1).
Overlay histogram showing THP-1 cells stained with A09775-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKN3 Antibody (A09775-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PKN3 (Source: Uniprot.org, NCBI)
Gene Name
PKN3
Full Name
Serine/threonine-protein kinase N3
Weight
99 kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.13; PKNBETA; protein kinase N3; Protein kinase PKN-beta; Protein-kinase C-related kinase 3; RP11-545E17.1; serine/threonine-protein kinase N3 PKN3 UTDP4-1 protein kinase N3 serine/threonine-protein kinase N3|protein kinase PKN-beta|protein-kinase C-related kinase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PKN3, check out the PKN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PKN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKN3 Antibody Picoband® (A09775-1)
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