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Product Info Summary
| SKU: | M03292 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-PKC eta Rabbit Monoclonal Antibody
SKU/Catalog Number
M03292
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKC eta Rabbit Monoclonal Antibody catalog # M03292. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC eta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03292)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADAH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKC eta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03292 is reactive to PRKCH in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
58206 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03292 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of PKC eta expression in MCF7 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For PRKCH (Source: Uniprot.org, NCBI)
Gene Name
PRKCH
Full Name
Protein kinase C eta type
Weight
58206 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein disulfide-isomerase A2;5.3.4.1;Pancreas-specific protein disulfide isomerase;PDIp;PDIA2;PDIP; PRKCH PKC-L, PKCL, PRKCL, nPKC-eta protein kinase C eta protein kinase C eta type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCH, check out the PRKCH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC eta Rabbit Monoclonal Antibody (M03292)
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Customer Q&As
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4 Customer Q&As for Anti-PKC eta Rabbit Monoclonal Antibody
Question
What sample was used in your WB analysis of M03292?
Verified customer
Asked: 2020-01-07
Answer
For the Anti-PKC Eta Rabbit Monoclonal Antibody (M03292), Western Blot analysis of PKC eta expression in MCF7 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
See below the WB image, lot number and protocol we used for thalamus using anti-PKC eta Monoclonal antibody M03292. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-20
Question
My question regarding product M03292, anti-PKC eta Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03292 anti-PKC eta Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-19
Question
I am interested in to test anti-PKC eta Monoclonal antibody M03292 on rat thalamus for research purposes, then I may be interested in using anti-PKC eta Monoclonal antibody M03292 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
The products we sell, including anti-PKC eta Monoclonal antibody M03292, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-19
