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Product Info Summary
| SKU: | A15347-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PITHD1 Antibody Picoband®
SKU/Catalog Number
A15347-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PITHD1 Antibody Picoband® catalog # A15347-1. Tested in WB, IHC, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PITHD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15347-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PITHD1 recombinant protein (Position: C12-S211). Human PITHD1 shares 98% amino acid (aa) sequence identity with mouse PITHD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15347-1 is reactive to PITHD1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.178kDa
Background of PITHD1
PITHD1 (PITH (C-terminal proteasome-interacting domain of thioredoxin-like) domain containing 1), also known as HT014, TXNL1CL or C1orf128, is a 211 amino acid nuclear protein containing one PITH domain. PITHD1 ectopic expression is thought to promote megakaryocytic differentiation and increase RUNX1 expression, while knockdown of PITHD1 was observed to have the opposite effect on both differentiation and RUNX1 expression. PITHD1 may regulate RUNX1 expression in two distinct fashions, by increasing transcription activity of proximal promoter and enhancing translational activity of an IRES element. Altered gene expression of the PITHD1 gene has been associated with leukemia development, with significant downregulation of expression observed in leukemic samples. The PITHD1 gene maps to chromosome 1 and is conserved in chimpanzee, Rhesus monkey, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C. e
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15347-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human Hela whole cell, human Jurkat whole cell, rat thymus tissue, mouse thymus tissue
IHC: human ganglioneuroma tissue, human ganglioneuroma tissue, human lung adenocarcinoma tissue, human lung adenocarcinoma tissue, human prostate adenocarcinoma tissue, human prostate adenocarcinoma tissue, human rectum tissue, human rectum tissue, human rectum adenocarcinoma tissue, human rectum adenocarcinoma tissue, human testicular seminoma tissue, human testicular seminoma tissue
ICC/IF: U2OS cell
FCM: HEL cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITHD1 antigen affinity purified polyclonal antibody (Catalog # A15347-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITHD1 at approximately 24 kDa. The expected band size for PITHD1 is at 24 kDa.
Click image to see more details
Figure 2. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human ganglioneuroma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human ganglioneuroma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 10. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 11. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 12. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 13. IHC analysis of PITHD1 using anti-PITHD1 antibody (A15347-1).
PITHD1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITHD1 Antibody (A15347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 14. IF analysis of PITHD1 using anti-PITHD1 antibody (A15347-1) and anti-Beta Tubulin antibody (M01857-3).
PITHD1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PITHD1 Antibody (A15347-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 15. Flow Cytometry analysis of HEL cells using anti-PITHD1 antibody (A15347-1).
Overlay histogram showing HEL cells stained with A15347-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITHD1 Antibody (A15347-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PITHD1 (Source: Uniprot.org, NCBI)
Gene Name
PITHD1
Full Name
PITH domain-containing protein 1
Weight
24.178kDa
Superfamily
PITHD1 family
Alternative Names
AD039; C1orf128; HT014; PITH (C-terminal proteasome-interacting domain of thioredoxin-like) domain containing 1; PITH domain-containing protein 1; PP603; RP5-886K2.4; TXNL1CL PITHD1 C1orf128, HT014, TXNL1CL PITH domain containing 1 PITH domain-containing protein 1|PITH (C-terminal proteasome-interacting domain of thioredoxin-like) domain containing 1|TXNL1 C-terminal like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PITHD1, check out the PITHD1 Infographic
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In this infographic, you will see the following information for PITHD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PITHD1 Antibody Picoband® (A15347-1)
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