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Product Info Summary
| SKU: | M05181-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PIN4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05181-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-PIN4 Rabbit Monoclonal Antibody catalog # M05181-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIN4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05181-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
29P19
Isotype
IgG
Immunogen
A synthesized peptide derived from human PIN4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05181-1 is reactive to PIN4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
14 kDa
Calculated molecular weight
62631 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05181-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, rat liver tissue, rat brain tissuemouse liver tissuemouse brain tissue
IHC: human prostate cancer tissue, human prostate cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PIN4 using anti-PIN4 antibody (M05181-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat brain tissue lysates.
Lane 5: mouse liver tissue lysates.
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIN4 antigen affinity purified monoclonal antibody (Catalog # M05181-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIN4 at approximately 14 kDa. The expected band size for PIN4 is at 14 kDa.
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Figure 2. IHC analysis of PIN4 using anti-PIN4 antibody (M05181-1).
PIN4 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIN4 Antibody (M05181-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of PIN4 using anti-PIN4 antibody (M05181-1).
PIN4 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIN4 Antibody (M05181-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PIN4 (Source: Uniprot.org, NCBI)
Gene Name
PIN4
Full Name
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
Weight
62631 MW
Superfamily
PpiC/parvulin rotamase family
Alternative Names
Transcription factor 7-like 1;HMG box transcription factor 3;TCF-3;TCF7L1;TCF3; PIN4 EPVH, PAR14, PAR17 peptidylprolyl cis/trans isomerase, NIMA-interacting 4 peptidyl-prolyl cis-trans isomerase NIMA-interacting 4|PPIase PIN4|eukaryotic parvulin homolog|hEPVH|hPar14|hPar17|parvulin|parvulin-14|parvulin-17|peptidyl-prolyl cis-trans isomerase Pin4|peptidyl-prolyl cis/trans isomerase EPVH|protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)|rotamase PIN4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIN4, check out the PIN4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIN4 Rabbit Monoclonal Antibody (M05181-1)
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