Anti-PIK3R6 Antibody Picoband™

Product Name

Anti-PIK3R6 Antibody Picoband™

View all Pik3r6 Antibodies

SKU/Catalog Number

A14338-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PIK3R6 Antibody Picoband™ catalog # A14338-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PIK3R6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14338-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PIK3R6 recombinant protein (Position: M1-Q537).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A14338-1 is reactive to Pik3r6 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

84 kDa

Calculated molecular weight

64099 MW

Background of Pik3r6

Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 7. The orthologous protein in the mouse binds to both the catalytic subunit and to G(beta/gamma), and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14338-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PIK3R6 using anti-PIK3R6 antibody (A14338-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R6 antigen affinity purified polyclonal antibody (Catalog # A14338-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R6 at approximately 84 kDa. The expected band size for PIK3R6 is at 84 kDa.

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Figure 2. Flow Cytometry analysis of HEL cells using anti-PIK3R6 antibody (A14338-1).
Overlay histogram showing HEL cells stained with A14338-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIK3R6 Antibody (A14338-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Pik3r6 (Source: Uniprot.org, NCBI)

Alternative Names

C17orf38; chromosome 17 open reading frame 38; FLJ34500; HsT41028; p84; p87 phosphoinositide 3-kinase gamma (PI3Kg) adapter protein; p87 PI3K adapter protein; p87(PIKAP); p87PIKAPDKFZp666P158; Phosphoinositide 3-kinase gamma adapter protein of 87 kDa; phosphoinositide 3-kinase regulatory subunit 6; phosphoinositide-3-kinase, regulatory subunit 6; PI3Kgamma adapter protein of 87 kDa Pik3r6|BB220380, p84, p84 PIKAP, p87, p87(PIKAP), p87PIKAP|phosphoinositide-3-kinase regulatory subunit 5|phosphoinositide 3-kinase regulatory subunit 6|p84 PI3K adapter protein|p87 phosphoinositide 3-kinase gamma (PI3Kg) adapter protein|phosphoinositide 3-kinase gamma adapter protein of 87 kDa|phosphoinositide-3-kinase, regulatory subunit 6

For more info on Pik3r6, check out the Pik3r6 Infographic

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In this infographic, you will see the following information for Pik3r6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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