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Product Info Summary
| SKU: | M05881 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Piccolo Monoclonal Antibody
SKU/Catalog Number
M05881
Size
100ug
Form
liquid
Description
Boster Bio Anti-Piccolo Monoclonal Antibody catalog # M05881. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Piccolo Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05881)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
6H9-B6
Isotype
IgG2b Kappa
Immunogen
Human full length recombinant protein (GenBank ID: BC001304.1)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~550kDa. No cross-reactivity to Bassoon.
Reactive Species
M05881 is reactive to PCLO in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
30063 MW
Background of Piccolo
Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05881 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Figure 2. Western blot analysis of Pclo using anti-Pclo antibody (M05881).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pclo antigen affinity purified polyclonal antibody (Catalog # M05881) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Pclo.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (M05881) . Tissue: Neuroblastoma cell line (SK-N-BE) . Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (M05881) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Piccolo Antibody. (D) Composite.
Protein Target Info & Infographic
Gene/Protein Information For PCLO (Source: Uniprot.org, NCBI)
Gene Name
PCLO
Full Name
Protein piccolo
Weight
30063 MW
Alternative Names
ACZ, Aczonin, PCLO, Pico, presynaptic cytomatrix protein Pclo|Ac, Acz, P, Pico|piccolo (presynaptic cytomatrix protein)|protein piccolo|aczonin|brain-derived HLMN protein|multidomain presynaptic cytomatrix protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCLO, check out the PCLO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCLO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Piccolo Monoclonal Antibody (M05881)
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Customer Q&As
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3 Customer Q&As for Anti-Piccolo Monoclonal Antibody
Question
We are currently using anti-Piccolo Monoclonal antibody M05881 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The anti-Piccolo Monoclonal antibody (M05881) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-15
Question
Is a blocking peptide available for product anti-Piccolo Monoclonal antibody (M05881)?
T. Carter
Verified customer
Asked: 2017-03-10
Answer
We do provide the blocking peptide for product anti-Piccolo Monoclonal antibody (M05881). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-03-10
Question
I see that the anti-Piccolo Monoclonal antibody M05881 works with IF, what is the protocol used to produce the result images on the product page?
J. Roberts
Verified customer
Asked: 2015-12-15
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-12-15
