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Product Info Summary
| SKU: | M00584S556 |
|---|---|
| Size: | 100 µl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-ULK1 (S556) Rabbit Monoclonal Antibody
SKU/Catalog Number
M00584S556
Size
100 µl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-ULK1 (S556) Rabbit Monoclonal Antibody catalog # M00584S556. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-ULK1 (S556) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00584S556)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
31U30
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-ULK1 (S556)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00584S556 is reactive to ULK1 in Human, Mouse
Reconstitution
Observed Molecular Weight
150 kDa
Calculated molecular weight
112.631kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00584S556 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000 ICC/IF 1:50-1:200
Positive Control
WB: human 293T whole cell, human MCF-7 whole cell, human A549 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ULK1 using anti-ULK1 antibody (M00584S556).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK1 antigen affinity purified monoclonal antibody (Catalog # M00584S556) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK1 at approximately 150 kDa. The expected band size for ULK1 is at 113 kDa.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ULK1 (Source: Uniprot.org, NCBI)
Gene Name
ULK1
Full Name
Serine/threonine-protein kinase ULK1
Weight
112.631kDa
Superfamily
protein kinase superfamily
Alternative Names
ATG1 autophagy related 1 homolog; ATG1; ATG1A; EC 2.7.11; EC 2.7.11.1; FLJ38455; FLJ46475; KIAA0722; serine/threonine-protein kinase ULK1; unc-51 (C. elegans)-like kinase 1; UNC51; Unc51.1; unc-51-like kinase 1 (C. elegans); Unc-51-like kinase 1 ULK1 ATG1, ATG1A, UNC51, Unc51.1, hATG1 unc-51 like autophagy activating kinase 1 serine/threonine-protein kinase ULK1|ATG1 autophagy related 1 homolog|autophagy-related protein 1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ULK1, check out the ULK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ULK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ULK1 (S556) Rabbit Monoclonal Antibody (M00584S556)
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