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Product Info Summary
| SKU: | A00039S811 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Rb (S811) RB1 Antibody
SKU/Catalog Number
A00039S811
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Rb (S811) RB1 Antibody catalog # A00039S811. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Rb (S811) RB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00039S811)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser811. AA range:771-820
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00039S811 is reactive to RB1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
106159 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00039S811 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Retinoblastoma (Phospho-Ser811) antibody
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Immunofluorescence analysis of HUVEC cells, using Retinoblastoma (Phospho-Ser811) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from K562 cells, using Retinoblastoma (Phospho-Ser811) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For RB1 (Source: Uniprot.org, NCBI)
Gene Name
RB1
Full Name
Retinoblastoma-associated protein
Weight
106159 MW
Superfamily
retinoblastoma protein (RB) family
Alternative Names
Retinoblastoma-associated protein;p105-Rb;pRb;Rb;pp110;RB1; RB1 OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110 RB transcriptional corepressor 1 retinoblastoma-associated protein|GOS563 exon 17 substitution mutation causes premature stop|exon 17 tumor GOS561 substitution mutation causes premature stop|prepro-retinoblastoma-associated protein|protein phosphatase 1, regulatory subunit 130|retinoblastoma 1|retinoblastoma suspectibility protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RB1, check out the RB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Rb (S811) RB1 Antibody (A00039S811)
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