Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody

Product Name

Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody

View all Raf-1 Antibodies

SKU/Catalog Number

P00446-5

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody catalog # P00446-5. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00446-5)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IOH-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-Raf1 (S259)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00446-5 is reactive to RAF1 in Human, Mouse, Rat

Applications

P00446-5 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

18 kDa

Calculated molecular weight

73052 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:100

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.

Gene/Protein Information For RAF1 (Source: Uniprot.org, NCBI)

Alternative Names

cRaf; c-Raf; c-Raf-1; CRAFraf proto-oncogene serine/threonine protein kinase; EC 2.7.11; EC 2.7.11.1; NS5; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; RAF; Raf1; Raf-1; Raf-1Oncogene RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; v-Raf-1 RAF1 CMD1NN, CRAF, NS5, Raf-1, c-Raf Raf-1 proto-oncogene, serine/threonine kinase RAF proto-oncogene serine/threonine-protein kinase|C-Raf proto-oncogene, serine/threonine kinase|Oncogene RAF1|proto-oncogene c-RAF|raf proto-oncogene serine/threonine protein kinase|v-raf-1 murine leukemia viral oncogene homolog 1|v-raf-1 murine leukemia viral oncogene-like protein 1

For more info on RAF1, check out the RAF1 Infographic

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In this infographic, you will see the following information for RAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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