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Product Info Summary
| SKU: | P00446-5 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00446-5
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody catalog # P00446-5. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00446-5)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IOH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Raf1 (S259)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00446-5 is reactive to RAF1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
18 kDa
Calculated molecular weight
73052 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00446-5 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.
Protein Target Info & Infographic
Gene/Protein Information For RAF1 (Source: Uniprot.org, NCBI)
Gene Name
RAF1
Full Name
RAF proto-oncogene serine/threonine-protein kinase
Weight
73052 MW
Superfamily
protein kinase superfamily
Alternative Names
RAF proto-oncogene serine/threonine-protein kinase;2.7.11.1;Proto-oncogene c-RAF;cRaf;Raf-1;RAF1;RAF; RAF1 CMD1NN, CRAF, NS5, Raf-1, c-Raf Raf-1 proto-oncogene, serine/threonine kinase RAF proto-oncogene serine/threonine-protein kinase|C-Raf proto-oncogene, serine/threonine kinase|Oncogene RAF1|proto-oncogene c-RAF|raf proto-oncogene serine/threonine protein kinase|v-raf-1 murine leukemia viral oncogene homolog 1|v-raf-1 murine leukemia viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAF1, check out the RAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody (P00446-5)
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