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Product Info Summary
| SKU: | P30439 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) PRKD1 Antibody
SKU/Catalog Number
P30439
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) PRKD1 Antibody (Catalog# P30439). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) PRKD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30439)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PKD1/2/3/PKC mu around the phosphorylation site of Ser738 and Ser742. Immunogen sequence location: 706-755
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P30439 is reactive to PRKD1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
101704 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30439 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30' and HT29 cells treated with serum 20% 15', using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human heart, using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For PRKD1 (Source: Uniprot.org, NCBI)
Gene Name
PRKD1
Full Name
Serine/threonine-protein kinase D1
Weight
101704 MW
Superfamily
protein kinase superfamily
Alternative Names
PRKD1;PKD;PKD1;PRKCM;Serine/threonine-protein kinase D1;Protein kinase C mu type;Protein kinase D;nPKC-D1;nPKC-mu;PRKD2;PKD2;HSPC187;Serine/threonine-protein kinase D2;nPKC-D2;PRKD3;EPK2;PRKCN;Serine/threonine-protein kinas PRKD1 CHDED, PKC-MU, PKCM, PKD, PRKCM protein kinase D1 serine/threonine-protein kinase D1|nPKC-D1|nPKC-mu|protein kinase C mu type|protein kinase C, mu|protein kinase D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKD1, check out the PRKD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) PRKD1 Antibody (P30439)
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No publications found for P30439
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) PRKD1 Antibody
Question
I see that the anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) antibody P30439 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder using anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) antibody P30439. Let me know if you need anything else.
B. Williams
Verified customer
Asked: 2016-06-09
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-09
Question
We are currently using anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) antibody P30439 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
S. Roberts
Verified customer
Asked: 2016-02-02
Answer
The anti-Phospho PKD1/2/3/PKC mu (Ser738+Ser742) antibody (P30439) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-02
