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Product Info Summary
| SKU: | P01883-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-NMDA Receptor NR2B Subunit (Tyr1336) Grin2b Antibody
SKU/Catalog Number
P01883-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-NMDA Receptor NR2B Subunit (Tyr1336) Grin2b Antibody (Catalog # P01883-2). Tested in WB, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-NMDA Receptor NR2B Subunit (Tyr1336) Grin2b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01883-2)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01883-2 is reactive to GRIN2B in Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
166071 MW
Background of GRIN2B
The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr-1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01883-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC: 1:400
ICC: 1:100
Validation Images & Assay Conditions
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Immunostaining of mouse dentate gyrus 48 hour post TMT treatment showing NR2B when phosphorylated at Tyr1336 in green and nuclei in blue.
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1336 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Protein Target Info & Infographic
Gene/Protein Information For GRIN2B (Source: Uniprot.org, NCBI)
Gene Name
GRIN2B
Full Name
Glutamate receptor ionotropic, NMDA 2B
Weight
166071 MW
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody GRIN2B DEE27, EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, NR3, hNR3 glutamate ionotropic receptor NMDA type subunit 2B glutamate receptor ionotropic, NMDA 2B|N-methyl D-aspartate receptor subtype 2B|N-methyl-D-aspartate receptor subunit 3|glutamate [NMDA] receptor subunit epsilon-2|glutamate receptor subunit epsilon-2|glutamate receptor, ionotropic, N-methyl D-aspartate 2B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIN2B, check out the GRIN2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIN2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-NMDA Receptor NR2B Subunit (Tyr1336) Grin2b Antibody (P01883-2)
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