Click image to see more details
Product Info Summary
| SKU: | P01883-4 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody
SKU/Catalog Number
P01883-4
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody (Catalog # P01883-4). Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01883-4)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01883-4 is reactive to GRIN2B in Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
166071 MW
Background of GRIN2B
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser-1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01883-4 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Protein Target Info & Infographic
Gene/Protein Information For GRIN2B (Source: Uniprot.org, NCBI)
Gene Name
GRIN2B
Full Name
Glutamate receptor ionotropic, NMDA 2B
Weight
166071 MW
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody GRIN2B DEE27, EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, NR3, hNR3 glutamate ionotropic receptor NMDA type subunit 2B glutamate receptor ionotropic, NMDA 2B|N-methyl D-aspartate receptor subtype 2B|N-methyl-D-aspartate receptor subunit 3|glutamate [NMDA] receptor subunit epsilon-2|glutamate receptor subunit epsilon-2|glutamate receptor, ionotropic, N-methyl D-aspartate 2B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIN2B, check out the GRIN2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIN2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody (P01883-4)
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody
Question
I was wanting to use your anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) antibody for WB for mouse buccal mucosa cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse buccal mucosa cell identification?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
You can see on the product datasheet, P01883-4 anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse buccal mucosa cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-27
Question
Will anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) antibody P01883-4 work on primate WB with fetal brain?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Our lab technicians have not tested anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) antibody P01883-4 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) antibody P01883-4 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) antibody P01883-4. Let me know if you need anything else.
R. Edwards
Verified customer
Asked: 2013-05-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-05-13
