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Product Info Summary
| SKU: | P06026 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Net (Ser357) Polyclonal Antibody
SKU/Catalog Number
P06026
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Net (Ser357) Polyclonal Antibody (Catalog # P06026). Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Net (Ser357) Polyclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06026)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human Net around the phosphorylation site of S357.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P06026 is reactive to ELK3 in Human, Monkey, Mouse
Reconstitution
Calculated molecular weight
44240 MW
Background of ELK3
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P06026 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of COLO cells using Phospho-Net (S357) Polyclonal antibody
Protein Target Info & Infographic
Gene/Protein Information For ELK3 (Source: Uniprot.org, NCBI)
Gene Name
ELK3
Full Name
ETS domain-containing protein Elk-3
Weight
44240 MW
Superfamily
ETS family
Alternative Names
ETS domain-containing protein Elk-3;ETS-related protein ERP;ETS-related protein NET;Serum response factor accessory protein 2;SAP-2;SRF accessory protein 2;ELK3;NET, SAP2; ELK3 ERP, NET, SAP-2, SAP2 ETS transcription factor ELK3 ETS domain-containing protein Elk-3|ELK3, ETS transcription factor|ELK3, ETS-domain protein (SRF accessory protein 2)|ETS-related protein ERP|ETS-related protein NET|SRF accessory protein 2|serum response factor accessory protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELK3, check out the ELK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Net (Ser357) Polyclonal Antibody (P06026)
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No publications found for P06026
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-Net (Ser357) Polyclonal Antibody
Question
Do you require any kind of licensing agreement with the researcher who wants to evaluate a Boster polyclonal antibody for possible inclusion in a commercialize product?
Verified customer
Asked: 2022-07-05
Answer
Yes, we need to have a licensing agreement, for many purposes but primarily to ensure the continuous supply of this product, which the researcher would need. We should ask them to discuss more details including the expected demand of this antibody. Since this is a polyclonal antibody we need to produce ahead of time and validate to ensure it keeps working properly for the customer.
Boster Scientific Support
Answered: 2022-07-05
Question
My question regarding product P06026, anti-Phospho-Net (Ser357) Polyclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P06026 anti-Phospho-Net (Ser357) Polyclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Phospho-Net (Ser357) Polyclonal antibody P06026. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
I see that the anti-Phospho-Net (Ser357) Polyclonal antibody P06026 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-10
Question
We are currently using anti-Phospho-Net (Ser357) Polyclonal antibody P06026 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on canine tissues as well?
M. Jones
Verified customer
Asked: 2015-05-06
Answer
The anti-Phospho-Net (Ser357) Polyclonal antibody (P06026) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-06
