Product Info Summary
SKU: | A00284S536-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-N kappa-p65 (S536) RELA Antibody
SKU/Catalog Number
A00284S536-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-N kappa-p65 (S536) RELA Antibody catalog # A00284S536-2. Tested in ELISA, IP, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-N kappa-p65 (S536) RELA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00284S536-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human NFkappaB-p65 around the phosphorylation site of S536.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00284S536-2 is reactive to RELA in Human, Monkey, Mouse, Rat
Applications
A00284S536-2 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
60219 MW
Background of RELA
HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IP 1:200-500
ELISA 1:10000
Validation Images & Assay Conditions
![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 10 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 10](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-10.jpg)
Click image to see more details
Figure 10. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of mouse liver tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 11 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 11](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-11.jpg)
Click image to see more details
Figure 11. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of mouse lung tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 12 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 12](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-12.jpg)
Click image to see more details
Figure 12. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of mouse spleen tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 13 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 13](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-13.jpg)
Click image to see more details
Figure 13. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of rat lung tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 14 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 14](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-14.jpg)
Click image to see more details
Figure 14. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of rat spleen tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 15 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 15](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-15.jpg)
Click image to see more details
Figure 15. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of rat testis tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 2 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-2.jpg)
Click image to see more details
Figure 2. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of human-appendix tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 3 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-3.jpg)
Click image to see more details
Figure 3. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of human colon tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 4 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-4.jpg)
Click image to see more details
Figure 4. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of human lung cancer tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 5 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-5.jpg)
Click image to see more details
Figure 5. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of human lung tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 6 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-6.jpg)
Click image to see more details
Figure 6. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of human stomach cancer tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 7 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-7.jpg)
Click image to see more details
Figure 7. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of human uterus cancer tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 8 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-8.jpg)
Click image to see more details
Figure 8. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of human uterus tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 9 a00284s536 2 anti phospho n kappa p65 s536 rela antibody ihc testing 9](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-ihc-testing-9.jpg)
Click image to see more details
Figure 9. Immunohistochemistry validation of RELA using Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2).
Immunohistochemical analysis of mouse brain tissue. A00284S536-2 was diluted at 1:200 (4°C![a00284s536 2 anti phospho n kappa p65 s536 rela antibody wb testing 1 a00284s536 2 anti phospho n kappa p65 s536 rela antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00284s536-2-anti-phospho-n-kappa-p65-s536-rela-antibody-wb-testing-1.jpg)
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Figure 1. Western blotting validation for Anti-Phospho-N kappa-p65 (S536) RELA Antibody A00284S536-2
Western blot (WB) analysis of NFkappaB-p65 (phospho Ser536) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For RELA (Source: Uniprot.org, NCBI)
Gene Name
RELA
Full Name
Transcription factor p65
Weight
60219 MW
Alternative Names
NF kB p65; NFkB p65; NF-kB p65; p65; rela p65; RelA; MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65 flow cytometry; p65 immunohistochemistry; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight po RELA CMCU, NFKB3, p65 RELA proto-oncogene, NF-kB subunit transcription factor p65|NF-kappa-B p65delta3|NF-kappa-B transcription factor p65|nuclear factor NF-kappa-B p65 subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RELA, check out the RELA Infographic
![RELA infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RELA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-N kappa-p65 (S536) RELA Antibody (A00284S536-2)
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