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Product Info Summary
| SKU: | P00535 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00535
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody catalog # P00535. Tested in WB, IP applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00535)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27M64
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-MLKL (S345)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00535 is reactive to Mlkl in Mouse
Reconstitution
Observed Molecular Weight
54 kDa
Calculated molecular weight
73052 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00535 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: L929
Validation Images & Assay Conditions
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Western blot analysis of Phospho-MLKL (S345) expression in (1) L929 treated with Z-VAD + TNF alpha + SM164 cell lysate; (2) Untreated.
Protein Target Info & Infographic
Gene/Protein Information For Mlkl (Source: Uniprot.org, NCBI)
Gene Name
Mlkl
Full Name
Mixed lineage kinase domain-like protein
Weight
73052 MW
Superfamily
protein kinase superfamily
Alternative Names
RAF proto-oncogene serine/threonine-protein kinase;2.7.11.1;Proto-oncogene c-RAF;cRaf;Raf-1;RAF1;RAF; Mlkl|9130019I15Rik|mixed lineage kinase domain-like|mixed lineage kinase domain-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mlkl, check out the Mlkl Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mlkl: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody (P00535)
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