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Product Info Summary
| SKU: | A11637T17S18 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Phospho-MLC-2 (T17/S18) MYL12A Antibody
SKU/Catalog Number
A11637T17S18
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-MLC-2 (T17/S18) MYL12A Antibody catalog # A11637T17S18. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MLC-2 (T17/S18) MYL12A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11637T17S18)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18. AA range:5-54
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11637T17S18 is reactive to MYL12A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
19794 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11637T17S18 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MLC-2 (Phospho-Thr17+Ser18) antibody
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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For MYL12A (Source: Uniprot.org, NCBI)
Gene Name
MYL12A
Full Name
Myosin regulatory light chain 12A
Weight
19794 MW
Alternative Names
Myosin regulatory light chain 12A;Epididymis secretory protein Li 24;HEL-S-24;MLC-2B;Myosin RLC;Myosin regulatory light chain 2, nonsarcomeric;Myosin regulatory light chain MRLC3;MYL12A;MLCB, MRLC3, RLC; MYL12A HEL-S-24, MLC-2B, MLCB, MRCL3, MRLC3, MYL2B myosin light chain 12A myosin regulatory light chain 12A|epididymis secretory protein Li 24|myosin RLC|myosin regulatory light chain 2, nonsarcomeric|myosin regulatory light chain 3|myosin regulatory light chain MRLC3|myosin, light chain 12A, regulatory, non-sarcomeric|myosin, light polypeptide, regulatory, non-sarcomeric (20kD)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYL12A, check out the MYL12A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYL12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MLC-2 (T17/S18) MYL12A Antibody (A11637T17S18)
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