Click image to see more details
Product Info Summary
| SKU: | P01488-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-Met (c-Met) (Y1349) Rabbit Monoclonal Antibody
View all HGFR/c-MET Antibodies
SKU/Catalog Number
P01488-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-Met (c-Met) (Y1349) Rabbit Monoclonal Antibody catalog # P01488-1. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Met (c-Met) (Y1349) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01488-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22M56
Isotype
IgG
Immunogen
A synthesized peptide derived from human c-Met
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01488-1 is reactive to MET in Human
Reconstitution
Observed Molecular Weight
156 kDa
Calculated molecular weight
59140 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01488-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Met (c-Met) using anti-Met (c-Met) antibody (P01488-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Met (c-Met) antigen affinity purified monoclonal antibody (Catalog # P01488-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Met (c-Met) at approximately 156 kDa. The expected band size for Met (c-Met) is at 156 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MET (Source: Uniprot.org, NCBI)
Gene Name
MET
Full Name
Hepatocyte growth factor receptor
Weight
59140 MW
Superfamily
protein kinase superfamily
Alternative Names
Ribosomal protein S6 kinase beta-1;S6K-beta-1;S6K1;2.7.11.1;70 kDa ribosomal protein S6 kinase 1;P70S6K1;p70-S6K 1;Ribosomal protein S6 kinase I;Serine/threonine-protein kinase 14A;p70 ribosomal S6 kinase alpha;p70 S6 kinase alpha;p70 S6K-alpha;p70 S6KA;RPS6KB1;STK14A; MET AUTS9, DFNB97, HGFR, RCCP2, c-Met MET proto-oncogene, receptor tyrosine kinase hepatocyte growth factor receptor|HGF receptor|HGF/SF receptor|SF receptor|proto-oncogene c-Met|scatter factor receptor|tyrosine-protein kinase Met
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MET, check out the MET Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MET: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Met (c-Met) (Y1349) Rabbit Monoclonal Antibody (P01488-1)
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