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Product Info Summary
| SKU: | A02608Y185-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody
SKU/Catalog Number
A02608Y185-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody catalog # A02608Y185-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02608Y185-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185. AA range:151-200
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02608Y185-1 is reactive to MAPK8 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
48296 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02608Y185-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
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Immunofluorescence analysis of human-lung tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of human-stomach tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of mouse-kidneystomach tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Western Blot analysis of various cells using Phospho-JNK1/2/3 (Y185) polyclonal antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SAPK/JNK (Phospho-Tyr185) antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using SAPK/JNK (Phospho-Tyr185) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using SAPK/JNK (Phospho-Tyr185) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, JNK1/2/3 (Phospho- Tyr185) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Protein Target Info & Infographic
Gene/Protein Information For MAPK8 (Source: Uniprot.org, NCBI)
Gene Name
MAPK8
Full Name
Mitogen-activated protein kinase 8
Weight
48296 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 8;MAP kinase 8;MAPK 8;2.7.11.24 MAPK8 JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c mitogen-activated protein kinase 8 mitogen-activated protein kinase 8|JUN N-terminal kinase|MAP kinase 8|c-Jun N-terminal kinase 1|stress-activated protein kinase 1|stress-activated protein kinase 1c
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK8, check out the MAPK8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1)
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